Traditionally, a nitrocellulose membrane is used, although nylon or a positively charged nylon membrane may be used. Transfer the denatured DNA to the membrane. Be aware, however, that the procedure may also be hampered by fragments that are too small.īe sure to neutralize the acid after this step, or the base after the prior step if you don't depurinate.Ĥ. Depurination with HCl (about 0.2M HCl for 15 minutes) takes the purines out, cutting the DNA into smaller fragments. Fragments greater than 15 kb are hard to transfer to the blotting membrane. Only ssDNA can transfer.Ī depurination step is optional. Denature the DNA (usually while it is still on the gel).įor example, soak it in about 0.5M NaOH, which would separate double-stranded DNA into single-stranded DNA. Digest the DNA with an appropriate restriction enzyme.ģ. Let's look at this technique in greater detail.ġ. This diagram shows the basic steps involved in a Southern blot. Under optimal conditions, you can expect to detect 0.1 pg of the DNA for which you are probing. The amount of DNA needed for this technique is dependent on the size and specific activity of the probe. For example, Southern Blotting could be used to locate a particular gene within an entire genome. Southern blotting is designed to locate a particular sequence of DNA within a complex mixture. To oversimplify, DNA molecules are transferred from an agarose gel onto a membrane. Southern who developed this procedure at Edinburgh University in the 1970s. Get the free view of Chapter 4, Molecular Basis of Inheritance Biology 12th Standard HSC for Maharashtra State Board additional questions for Mathematics Biology 12th Standard HSC for Maharashtra State Board Maharashtra State Board,Īnd you can use to keep it handy for your exam preparation.Southern blotting was named after Edward M. Maximum Maharashtra State Board Biology 12th Standard HSC for Maharashtra State Board students prefer Balbharati Textbook Solutions to score more in exams. The questions involved in Balbharati Solutions are essential questions that can be asked in the final exam. Using Balbharati Biology 12th Standard HSC for Maharashtra State Board solutions Molecular Basis of Inheritance exercise by students is an easy way to prepare for the exams, as they involve solutionsĪrranged chapter-wise and also page-wise. Balbharati textbook solutions can be a core help for self-study and provide excellent self-help guidance for students.Ĭoncepts covered in Biology 12th Standard HSC for Maharashtra State Board Maharashtra State Board chapter 4 Molecular Basis of Inheritance are Deoxyribonucleic Acid (DNA) and Its Structure, The Genetic Material is a DNA, DNA Replication, Protein Synthesis, Regulation of Gene Expression, Operon Concept, Genomics, Human Genome Project, DNA Fingerprinting Technique, Packaging of DNA Helix. This will clear students' doubts about questions and improve their application skills while preparing for board exams.įurther, we at provide such solutions so students can prepare for written exams. The detailed, step-by-step solutions will help you understand the concepts better and clarify any confusion.īalbharati solutions for Mathematics Biology 12th Standard HSC for Maharashtra State Board Maharashtra State Board 4 (Molecular Basis of Inheritance) include all questions with answers and detailed explanations. has the Maharashtra State Board Mathematics Biology 12th Standard HSC for Maharashtra State Board Maharashtra State Board solutions in a manner that help students
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